ISO 17721-1-2021 pdf download.Quantitative determination of antibacterial activity of ceramic tile surfaces一Test methods一 Part 1: Ceramic tile surfaces with incorporated antibacterial agents. Other culture media may be selected based on the requirements for the bacteria selected for the testing. Details of the selected media shall be included in the test report under culture conditions. Prepared culture media may be stored at 5 °C – 10 °C for up to one month. 4.2.3 1/500 nutrient broth For 1 000 ml of purified water, take 3,0 g of meat extract, 10,0 g of peptone and 5,0 g of sodium chloride, put them in a flask and dissolve them thoroughly. Dilute this medium 500 times using distilled or deionized water, and set the p1-I to (7,0 ± 0,2) using hydrochloric acid solution or sodium hydroxide solution. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if 1/500 nutrient broth is not used immediately, store it at 5 °C to 10 °C. Other culture media may be selected based on the requirements for the bacteria selected for the testing. Details of the selected media shall be included in the test report under culture conditions. Prepared culture media may be stored at 5 °C – 10°C for up to one month. 4.2.4 Nutrient agar For 1 000 ml of purified water, take 3,0 g of meat extract, 5,0 g of peptone and 15,0 g of agar powder, put them in a flask and mix. Heat the flask in boiling water to dissolve the contents thoroughly. Use a 0.1 mol/l solution of sodium hydroxide to bring the pH to (6,8 ± 0,2) at 25 °C. Sterilize in an autoclave at 121 °C ± 2 °C for at least 15 mm. After preparation, if nutrient agar is not used immediately, store it at S °C to 10 °C. Keep the medium temperature between 45 °C and 48 °C when mixing with a bacterial suspension. Other culture media may be selected based on the requirements for the bacteria selected for the testing. Details of the selected media shall be included in the test report under culture conditions. Prepared culture media may be stored at 5 °C – 10 °C for up to one month. 4.2.5 Soybean-casein digest broth with lecithin and polysorbate 80 (SCDLP) For 1 000 ml of purified water, take 17.0 g of casein peptone, 3,0 g of...

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