ISO 23906-1-2020 pdf download.Cigarettes一Determination of benzo[a]pyrene in cigarette mainstream smoke with an intense smoking regime using GC/MS – Part 1: Method using methanol as extraction solvent. 6.2 Water, complying with grade 2 of Iso 3696 or better. 6.3 Cyclohexane, of known purity, not less than 99%, CAS 110-82-7. 6.4 Toluene, of known purity, not less than 99 %, CAS 108-88-3. 6.5 Benzolajpyrene, of known purity, not less than 98 %, CAS 50-32-8. 6.6 Benzolajpyrene.d12, of known purity, not less than 98%, CAS 63466-71-7. 6.7 Helium, carrier gas of known purity, not less than 99,999 %, CAS 7440-59-7. WARNING — Benzo[ajpyrene and benzo[ajpyrene-d12 are carcinogens. Appropriate safety precautions shall be taken when manipulating these compounds or any solution containing these corn pounds. 7 Standards 7.1 General Certified BIaJP or BLaJPd12 solutions can be used as reference material. 7.2 Primary BlaiP stock solution Dissolve 10 mg BIaJP, weighed to the nearest 0,01 mg, into a 10 ml volumetric flask and fill to the mark with toluene. 7.3 Secondary Blair stock solution Dilute I ml of the primary BjaJP stock solution (see 22) into a 100 ml volumetric flask and fill to the mark with methanol. 7.4 B[aJP-d12 stock solution Dissolve 10 mg B[aJP-d12, weighed to the nearest 0,01 mg, into a It) ml volumetric flask and fill to the mark with toluene. 7.5 BaJP-d12 spiking solution Using a gas syringe, transfer 100 ii of the B[aJP-d12 stock solution (see 7.4) into a 100 ml volumetric flask and fill to the mark with methanol. This solution has a mass concentration of approximately I pg/mi. 7.6 Working standard solutions Prepare six working standard solutions that cover the concentration range of interest. For example, transfer 20 p1 of the BIaJP-d12 stock solution (see 7.4) and 10 p1 to 2 000 p1 of the secondary B[a]P stock solution (see 7.3) into 100 ml volumetric flasks and fill to the mark with cyclohexane. These solutions have a mass concentration of approximately 0,2 pg/mI of B[a]Pd12 and mass concentrations from 1 ng/ ml to 200 ng/ml of B[a]P. 7,7 Storage of standard solutions The standard solutions (see 7.2 to 7.6) are stable for up to six months if stored below —18 °C. Storage in amber glassware and away from the light is recommended.ISO 23906-1 pdf download.
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