ISO/TS 23034-2021 pdf download.Nanotechnologies – Method to estimate cellular uptake of carbon nanomaterials using optical absorption.
This document describes a near-infrared optical absorption method to estimate the in vitro cellular uptake of carbon nanomaterials including both internalized and/or tightly adhered to the cell membrane from liquid dispersions. This is a simple method to screen carbon nanomaterials uptake; additional analysis using a different technique can be required if quantification is desired.
2 Normative references
The following document is referenced in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
ISO 3696:1987, Water for analytical laboratory use — Specification and test methods ISO/TS 80004-3, Nanotechnologies — Vocabulary — Part 3: Carbon nano-objects
3 Terms, definitions and abbreviated terms
For the purposes of this document, the following terms, definitions and abbreviations as well as the terms and definitions given in ISO/TS 80004-3 apply.
ISO and IEC maintain terminological databases for use in standardization at the following addresses:
— ISO Online browsing platform: available at https://www.iso.org/obp
— IEC Electroped ia: available at http://www.electropedia.org/
3.1 Terms and definitions
3.1.1 cellular uptake
internalization or association of a substance by a living cell
3.1.2 cell lysis
destruction or dissolution of cells with release of contents
3.1.3 absorbance
measure of the capacity of a substance to absorb light at a specified wavelength
3.2 Abbreviated terms
CNH carbon nanohoiii
CNM carbon nanomaterial
CNT carbon nanotube.ISO/TS 23034 pdf download.